Short version:

OneDrive for Business and SharePoint online upload limit increased from 100GB per file 250GB via the sync client as well as the web app. Increased capacity available now. See link for suggested considerations from Microsoft.

Details:

Previously, OneDrive for Business and SharePoint online customers had an upload limit for 100GB per file. And customers have been asking for increased upload limits. Now, customers can upload files up to 250GB across OneDrive for Business and SharePoint online. 

All users of OneDrive for Business and SharePoint online will be able to upload files sizes up to 250GB via the sync client as well as the web app.

Considerations from Microsoft:

If your tenant has large files that could not be uploaded previously, we recommend you test a few large files (sync client recommended for uploading large files) and review how to create large dummy files here.

Please keep in mind there are many factors that may impact your upload capability including;

  • bandwidth
  • location
  • other network traffic

Availability:

This feature has already rolled out.